Repetitive brief ischemia can promote bone healing in a rat tibia fracture model
نویسندگان
چکیده
Objective: To observe the effects of repetitive brief ischemia on promoting bone healing in a rat tibia fracture model. Methods: To achieve the aim of the study, rats with right tibia closed fractured were administered homemade tourniquet placed on right thigh 10 min inflated/10 min deflated 3 times ischemia treatment at three interval time (24 hours, 48 hours, and 72 hours) for 5 consecutive weeks after first ischemia treatment at 24 hours after bone fractured. Digital X-rays, computed tomography, serum insulin-like growth factors-2 (IGF-2), and Immunohistochemical Detection of IGF-2 in fractured bone area were detected. Results: The present data showed that repetitive brief ischemia treatment can increase the mean digital X-rays scores, BV, and TV and decrease the TMD and callus BMD. It can stimulate the secretion of serum IGF-2. Conclusion: Repetitive brief ischemia can promote bone healing by increasing the level of IGF-2 in a rat tibia fracture model.
منابع مشابه
The effects of melatonin and caffeic acid phenethyl ester (CAPE) on fracture healing under ischemic conditions.
OBJECTIVE The aim of this study was to investigate the effects of antioxidant molecules melatonin and caffeic acid phenethyl ester (CAPE) on fracture healing under ischemic conditions. METHODS A right tibia fracture was created and fixed with an intramedullary pin in forty four male Wistar-albino rats. The rats were then randomly allocated to fracture, fracture-ischemia, fracture- ischemia-me...
متن کاملTitle: Salvianolic acid B promotes bone formation by increasing activity of alkaline phosphatase in a rat tibia Fracture Model: a pilot study
Background Radix Salviae miltiorrhizae is a herb frequently used within traditional Chinese medicine for the treatment of cardiovascularand trauma-related diseases. Danshen is the dried root of Salviae miltiorrhizae, from which the polyphenolic compound Salvianolic acid B (Sal B) can be obtained. Sal B is a key component of Danshen. The aim of this study was to determine the effect of Sal B on ...
متن کاملThe development of a novel model of direct fracture healing in the rat
OBJECTIVES Small animal models of fracture repair primarily investigate indirect fracture healing via external callus formation. We present the first described rat model of direct fracture healing. METHODS A rat tibial osteotomy was created and fixed with compression plating similar to that used in patients. The procedure was evaluated in 15 cadaver rats and then in vivo in ten Sprague-Dawley...
متن کاملThe effect of prednisolone on the sensitivity of Technetium 99m-methylene diphosphonate ( 99m Tc-MDP) scintigraphy to detect simulated closed fracture in the rat tibia
Introduction: The purpose of the present study was to evaluate the effect of prednisolone on the sensitivity of bone scanning to detect the simulated closed fracture in the rat tibia. Methods: A total number of forty eight adult, male NMRI rats randomly assigned into two parts, one part for 4 and other for 8 weeks experiments. Each part has been divided into four groups, one group not receivi...
متن کاملSalvianolic acid B promotes bone formation by increasing activity of alkaline phosphatase in a rat tibia fracture model: a pilot study
BACKGROUND Radix Salviae miltiorrhizae is a herb frequently used within traditional Chinese medicine for the treatment of cardiovascular- and trauma-related diseases. Danshen is the dried root of Salviae miltiorrhizae, from which the polyphenolic compound Salvianolic acid B (Sal B) can be obtained. Sal B is a key component of Danshen. The aim of this study was to determine the effect of Sal B o...
متن کامل